Alpha amidating enzyme datingsite 30 plus

04-May-2020 23:35

Peptidylglycine α-amidating monooxygenase (PAM; EC 1.14.17.3) catalyzes the COOH-terminal α-amidation of peptidylglycine substrates, yielding amidated products.

We have previously reported a putative regulatory RNA binding protein (PAM m RNA-BP) that binds specifically to the 3′ untranslated region (UTR) of PAM-m RNA.

It associates predominantly with a short poly(U) sequence (UUU) at the 3′ end of almost all nascent Pol III transcripts (27, 47).The size of La varies from about 32 k Da in yeasts, where La has only one RRM, to 50 k Da in humans, where La has two RRMs.Both the La motif and the central RRM are necessary for high-affinity RNA binding (7, 17), and it has been suggested that the role of the La motif is to provide specific recognition for UUU sequences (45, 27).To further analyze the molecular mechanisms that are involved in PAM m RNA metabolism, our group identified a protein which binds specifically to a segment of the 3′ untranslated region (UTR) of PAM m RNA and named it the PAM m RNA-binding protein (PAM m RNA-BP) (16).Our study describes the isolation of the PAM m RNA-BP and demonstrates by several criteria that this protein is La antigen.

It associates predominantly with a short poly(U) sequence (UUU) at the 3′ end of almost all nascent Pol III transcripts (27, 47).

The size of La varies from about 32 k Da in yeasts, where La has only one RRM, to 50 k Da in humans, where La has two RRMs.

Both the La motif and the central RRM are necessary for high-affinity RNA binding (7, 17), and it has been suggested that the role of the La motif is to provide specific recognition for UUU sequences (45, 27).

To further analyze the molecular mechanisms that are involved in PAM m RNA metabolism, our group identified a protein which binds specifically to a segment of the 3′ untranslated region (UTR) of PAM m RNA and named it the PAM m RNA-binding protein (PAM m RNA-BP) (16).

Our study describes the isolation of the PAM m RNA-BP and demonstrates by several criteria that this protein is La antigen.

The elucidation of the mechanisms of regulation of PAM expression should bring new insights into the field of neuropeptide-processing enzyme regulation.